Kinetoplast DNA-encoded ribosomal protein S12

An intergenic G-rich region in Leishmania tarentolae kinetoplast maxicircle DNA is a pan-edited cryptogene encoding ribosomal protein S12.

Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added a...

Role of ribosomal protein S12 in discrimination of aminoacyl-tRNA.

Ribosomes of streptomycin-resistant strains (&r-R) translate genetic messages with fewer errors than ribosomes of streptomycin-sensitive (S&S) strains. I have examined whether the increased level of tRNA discrimination by &r-R ribosomes, relative to Str-S ribosomes, occurs in an initial discrimination step before GTP hydrolysis during enzymatic tRNA binding, or in a second discrimination, or pr...

Kinetoplast DNA Replication

The order Kinetoplastida is composed of several families of flagellated protozoa which are among the most ancient of the eukaryotes. The most prominent members of this order are parasites that are responsible for major tropical diseases. These parasites include Trypanosoma brucei, the African trypanosome; Trypanosoma cruzi, the South American trypanosome; and several species of Leishmania. Othe...

RNAi libraries and kinetoplast DNA.

African trypanosomes have a remarkable mitochondrial DNA termed kDNA (kinetoplast DNA) that contains several thousands of topologically interlocked DNA rings. Because of its highly unusual structure, kDNA has a complex replication mechanism. Our approach to understanding this mechanism is to identify the proteins involved and to characterize their function. So far approx. 30 candidate proteins ...

Improvement of -Amylase Production by Modulation of Ribosomal Component Protein S12 in Bacillus subtilis 168